1P257 Proton transfer in Photoactive Yellow Protein(PYP)
نویسندگان
چکیده
منابع مشابه
Signal transduction in the photoactive yellow protein. II. Proton transfer initiates conformational changes.
Molecular dynamics simulation techniques, together with semiempirical PM3 calculations, have been used to investigate the effect of photoisomerization of the 4-hydroxy-cinnamic acid chromophore on the structural properties of the photoactive yellow protein (PYP) from Ectothiorodospira halophila. In this bacteria, exposure to blue light leads to a negative photoactic response. The calculations s...
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Photoactive yellow protein (PYP) is the protein responsible for the negative phototaxis of Halorhodospira halophila. The absorption of blue light of its chromophore, p-coumaric acid, triggers a photocycle in PYP, initiated by trans-cis isomerization of its chromophore. Pump-probe spectroscopy in the visible reveals that proline at position 68 is responsible for the high efficiency of isomerizat...
متن کاملSubpicosecond Excited-State Proton Transfer Preceding Isomerization During the Photorecovery of Photoactive Yellow Protein.
The ultrafast excited-state dynamics underlying the receptor state photorecovery is resolved in the M100A mutant of the photoactive yellow protein (PYP) from Halorhodospira halophila. The M100A PYP mutant, with its distinctly slower photocycle than wt PYP, allows isolation of the pB signaling state for study of the photodynamics of the protonated chromophore cis-p-coumaric acid. Transient absor...
متن کاملConcerted motions in the photoactive yellow protein.
Molecular dynamics simulations have been performed with the aim of identifying concerted backbone motions in the photoactive yellow protein. Application of the essential dynamics method revealed large, chromophore-linked fluctuations of the protein in the ground state, as well as in a form containing the isomerized chromophore. Various loops become more mobile upon isomerization of the chromoph...
متن کاملCorrigendum: Controlling Radical Formation in the Photoactive Yellow Protein Chromophore.
To understand how photoactive proteins function, it is necessary to understand the photoresponse of the chromophore. Photoactive yellow protein (PYP) is a prototypical signaling protein. Blue light triggers trans-cis isomerization of the chromophore covalently bound within PYP as the first step in a photocycle that results in the host bacterium moving away from potentially harmful light. At hig...
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ژورنال
عنوان ژورنال: Seibutsu Butsuri
سال: 2005
ISSN: 0582-4052,1347-4219
DOI: 10.2142/biophys.45.s96_1